Τίτλος:	Expression profile of CYP1A1 and CYP1B1 enzymes in colon and bladder tumors.
Δημιουργός:	Androutsopoulos, VP Spyrou, I Ploumidis, A Papalampros, AE Kyriakakis, M Delakas, Dimitris Spandidos, Demetrios A. Tsatsakis, AM
Χρονολογία:	2013
Γλώσσα:	Αγγλικά
Εξειδίκευση τύπου :	Άρθρο
Περιγραφή:	PLoS One. 2013 Dec 16;8(12):e82487
Περίληψη:	BACKGROUND: The cytochrome P450 CYP1A1 and CYP1B1 enzymes are involved in carcinogenesis via activation of pro-carcinogenic compounds to carcinogenic metabolites. CYP1A1 and CYP1B1 have shown elevated levels in human tumors as determined by qRT-PCR and immunohistochemical studies. However studies that have examined CYP1 expression by enzyme activity assays are limited. RESULTS: In the current study the expression of CYP1A1 and CYP1B1 was investigated in a panel of human tumors of bladder and colorectal origin by qRT-PCR and enzyme activity assays. The results demonstrated that 35% (7/20) of bladder tumors and 35% (7/20) of colon tumors overexpressed active CYP1 enzymes. CYP1B1 mRNA was overexpressed in 65% and 60% of bladder and colon tumors respectively, whereas CYP1A1 was overexpressed in 65% and 80% of bladder and colon tumors. Mean mRNA levels of CYP1B1 and CYP1A1 along with mean CYP1 activity were higher in bladder and colon tumors compared to normal tissues (p<0.05). Statistical analysis revealed CYP1 expression levels to be independent of TNM status. Moreover, incubation of tumor microsomal protein in 4 bladder and 3 colon samples with a CYP1B1 specific antibody revealed a large reduction (72.5 ± 5.5 % for bladder and 71.8 ± 7.2% for colon) in catalytic activity, indicating that the activity was mainly attributed to CYP1B1 expression. CONCLUSIONS: The study reveals active CYP1 overexpression in human tumors and uncovers the potential use of CYP1 enzymes and mainly CYP1B1 as targets for cancer therapy.
Θέμα:	Colonic Neoplasms Urinary Bladder Neoplasms Cytochrome P-450 CYP1A1 Cytochrome P-450 CYP1B1 Cytochromes Κυτοχρώματα Παχέος Εντέρου Νεοπλάσματα Ουροδόχου Κύστεως Νεοπλάσματα
Εναλλακτικός δικτυακός τόπος:	http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3864999/pdf/pone.0082487.pdf
Άδεια Χρήσης:	Αναφορά Παρόμοια Διανομή
Διάθεση ψηφιακού τεκμηρίου:	Πλήρης
Εμφανίζεται στις Ομάδες Τεκμηρίων:	Ουρολογία

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